Biologics
Characterization of biologics
Biologics and their biosimilars comprise the cutting-edge of biopharmaceutical products such as antibodies, enzymes, AAVs, vaccines or hormones used to treat many complex conditions, including cancer, neurologic and autoimmune disorders, and diabetes. Biologics are far from easy to characterize as they are highly complex mixtures of biological compounds produced by natural sources like cell cultures or microorganisms.
We at Biofidus are well experienced with a wide variety of biologics. Through hundreds of projects with small and big pharma clients we were able to build up broad knowledge in characterizing different classes of biologics including IgGs, bispecific antibodies, nanobodies, antibody-drug conjugates (ADCs), fusion proteins and vector-based therapeutics. From structural and functional characterization, from the identification of impurities to complex comparability or forced degradation studies.
Get in contact with us to find the optimal strategy for your project and to speak directly from expert to expert.
Standalone and integral bioanalytical services for:
Tell us about your project
We‘ll advice you to define your assay needs
Technologies used
- LC-ESI-MS/MS peptide mapping
- LC-ESI-MS intact mass analysis
- Reversed phase (RP) chromatography
- Size exclusion chromatography (SEC)
- Hydrophilic interaction chromatography (HILIC)
- Hydrophobic interaction chromatography (HIC)
- Ion exchange chromatography (IEX)
- High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
- Absolute protein quantification (AQUA)
- Capillary gel electrophoresis (CGE)
- Capillary isoelectric focusing (cIEF)
- Capillary zone electrophoresis (CZE)-UV
- (2D-)SDS-PAGE
- Gel based isoelectric focusing (IEF)
- Western blot
- ELISA
- Surface plasmon resonance (SPR)
- Cell-based assays
- Plasma/serum stability assays
- Nanopore DNA/RNA sequencing
- qPCR
- Analytical ultracentrifugation (AUC)
- Circular dichroism (CD)
- Differential scanning calorimetry (DSC)
- Dynamic light scattering (DLS)
- Fourier transform infrared (FTIR) spectroscopy
Our CEO Dr. Benjamin Müller is your contact person for all requests concerning the general topic of Biologics.
Phone: +49 (0)521 329 363 41
Mail: Benjamin.Mueller@Biofidus.com