
Fusion Proteins
Characterization of fusion proteins
The remarkable features of fusion proteins to combine the specific properties of different proteins into a single biomolecule led to completely novel and highly targeted therapeutic approaches. Since several years, biologics such as Etanercept and Aflibercept and their biosimilars have shown that the targeted combination of selected protein domains can lead to improved efficacy and stability of the naturally occurring proteins.
To ensure correct fusion and integrity of the desired protein, we at Biofidus offer a broad range of state-of-the-art analytical services, to accomplish the structural as well as the functional characterization of fusion proteins, allowing us to provide you with a suitable method setup for your analytical challenge.
Besides an in-depth analysis of the protein primary structure and post translational modifications we are experts in analyzing the functional properties of your lead molecules via customized cell-based assays and surface plasmon resonance (SPR) analysis.
Our vast experience and deep knowledge in this field allows us to guide you through the variety of available methods and help you find the optimal analytical solution for your project.
In accordance with your needs, we adapt and, if required, qualify (according to ICH guidelines) our platform methods tailored to your drug substance (DS), its individual formulation matrix and your distinct analytical question.
Get in contact with us to quickly find the optimal setup for your analytical challenge and to speak directly from expert to expert
Our capabilities
- Initial protein characterization
- Structural integrity
- Clone selection / lead selection
- Impurity characterization
- Forced degradation studies
- Stability testing
- Comparability/biosimilarity studies
- Formulation development
- Process characterization
- Setup, transfer and qualification of analytical methods
Tell us about your project
We‘ll advice you to define your assay needs
Technologies used
- LC-ESI-MS/MS peptide mapping
- LC-ESI-MS intact mass analysis
- Reversed phase (RP) chromatography
- Size exclusion chromatography (SEC)
- Hydrophilic interaction chromatography (HILIC)
- Hydrophobic interaction chromatography (HIC)
- Ion exchange chromatography (IEX)
- High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
- Absolute protein quantification (AQUA)
- Capillary gel electrophoresis (CGE)
- Capillary isoelectric focusing (cIEF)
- Capillary zone electrophoresis (CZE)-UV
- (2D-)SDS-PAGE
- Gel based isoelectric focusing (IEF)
- Western blot
- ELISA
- Surface plasmon resonance (SPR)
- Cell-based assays
- Plasma/serum stability assays
- Nanopore DNA/RNA sequencing
- qPCR
- Analytical ultracentrifugation (AUC)
- Circular dichroism (CD)
- Differential scanning calorimetry (DSC)
- Dynamic light scattering (DLS)
- Fourier transform infrared (FTIR) spectroscopy
Our CEO Dr. Benjamin Müller advices you on all your requests concerning the general topic of protein characterization.
Phone: +49 (0)521 329 363 41
Mail: Benjamin.Mueller@Biofidus.com
