AAV Characterization
AAV Characterization
The development of cell and gene-based therapeutics like AAV (adeno-associated virus) vectors has gained significant importance in recent years. We provide a comprehensive list of analytical methods for an in-depth characterization of your AAV construct. Through a targeted analysis setup of long read next-generation sequencing (ONT), quantitative PCR (qPCR), transmission electron microscopy (TEM) and LC-MS/MS techniques, we will provide you detailed information about the genome integrity, capsid surface and empty/full ratio characteristics of your product.
Our routine experts in sequencing and capsid protein characterization will guide you through the variety of available methods and help you find the optimal analytical solution for your project.
In accordance with your needs, we adapt and, if required, qualify (according to ICH guidelines) our platform methods tailored to your product, its individual formulation matrix and your distinct analytical question.
Get in contact with us to quickly find the optimal setup for your analytical challenge and to speak directly from expert to expert
Our capabilities
- Empty/full ratio determination
- Genome integrity analysis and sequence verification by long read sequencing
- Analysis of chimeric genome fusions and genome duplications
- ITR characterization
- Identification and quantification of host cell DNA impurities
- Capsid protein characterization including PTMs
- VP stoichiometry analysis
Standalone and integral bioanalytical services
- Post translational modifications (PTM) analysis of capsid proteins by LC-MS
- Amino acid sequence confirmation by LC-MS peptide mapping
- Ratio of cap proteins by LC-MS intact mass analysis or LC-MS peptide mapping
- Analysis of SNPs, genome duplications, genome truncations and chimeric genome fusions (with host cell DNA or E. coli DNA) via long read sequencing
- Genome heterogeneity, integrity and impurity analysis via long read sequencing
- Genomic titer by quantitative PCR (qPCR)
- Host cell protein (HCP) ELISA
- Absolute quantification (AQUA) of HCP by mass spectrometry
- Aggregation analysis by size exclusion chromatography (SEC)
- Characterization of empty/full ratio of your capsids by transmission electron microscopy (TEM) or HPLC
- Total virus particle titer by AAV capsid ELISA
- Total protein quantification via UV280nm or Bradford
- Sequence verification via long read sequencing
Tell us about your project
We‘ll advice you to define your assay needs
Technologies used
- LC-ESI-MS/MS peptide mapping
- LC-ESI-MS intact mass analysis
- Anion exchange chromatography (AEX)
- Absolute protein quantification (AQUA)
- Capillary gel electrophoresis (CGE)
- Western blot
- ELISA
- Plasma/serum stability assays
- Nanopore DNA/RNA sequencing
- Quantitative PCR (qPCR)
- Transmission electron microcopy (TEM)
Our Expert Tobias Luttermann advices you on for all inquiries related to AAVs and gene based therapies.
Phone: +49 (0)521 329 363 48
Mail: Tobias.Luttermann@Biofidus.com
