Spent Media Analysis

The analysis and understanding of small molecules is crucial to the production process of biopharmaceuticals or other molecules produced in eukaryotic or prokaryotic cells.

The analysis aids in detection of substrate limitations or the accumulation of cytotoxic molecules. At Biofidus we offer standardized assays for the analysis of more than 50 different media components.

  • Alanine
  • Arginine
  • Asparagine
  • Aspartic Acid
  • Cysteine/Cystine (quantified as one compound)
  • Glutamic Acid
  • Glutamine
  • Glycine
  • Histidine
  • Isoleucine
  • Leucine
  • Lysine
  • Methionine
  • Phenylalanine
  • Proline
  • Serine
  • Threonine
  • Tryptophan
  • Tyrosine
  • Valine

  • 2-Hydroxybutyric Acid
  • Alpha-Ketoglutaric Acid
  • Citric Acid
  • Fumaric Acid
  • Lactic Acid
  • Maleic Acid
  • Malic Acid
  • Pyroglutamic Acid
  • Pyruvic Acid
  • Succinic Acid
  • Etc.

  • Spermidine
  • Spermine
  • Putrescine

  • Fructose
  • Fucose
  • Galactose
  • Glucose
  • Lactose
  • Mannose
  • Raffinose
  • Sialic Acids
  • Sucrose
  • Xylose
  • Etc.

  • Biotin (B7)
  • Choline
  • Cyanocobalamin (B12)
  • Folic Acid (B9)
  • Niacin (B3)
  • Nicotinamide (B3)
  • Ca-Pantothenate (B5)
  • Pyridoxal (B6)
  • Pyridoxal-5-Phosphate (B6)
  • Pyridoxamine (B6)
  • Pyridoxine (B6)
  • Riboflavin (B2)
  • Thiamine (B1)

  • Dipeptides
  • Fatty acids
  • Surfactants
  • Polysorbate 20/80 (Tween)
  • Extractables & Leachables
  • Nucleobases

  • LC-UV
  • LC-PAD
  • LC-FLD
  • LC-QQQ-MS
  • LC-QTOF-MS
  • GC-MS on request

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