Protein Analysis

We are specialized in the characterization of biopharmaceutical proteins, examples include

  • monoclonal antibodies
  • Fc-fusion proteins
  • erythropoietin
  • (PEG)-G-CSF
  • ADCs

Our portfolio includes in depth analysis of the protein primary structure, amino acid modifications, as well as charge and size heterogeneity.

We are specialists in determining protein glycosylation structures, which are among the most important protein modifications that can have significant influence on function and stability of biopharmaceutical molecules.

Our vast experience and deep knowledge in this field allows us to guide you through the variety of available methods and help you find the optimal analytical solution for your project.

  • Access to amino acid sequence and N- & C-terminal truncation
  • Amino acid content after protein hydrolysis by LC-FLD incl. determination of extinction coefficient
  • Peptide mapping by (LC-)MALDI-TOF and LC-ESI-TOF
  • Peptide mapping by RP-UV
  • N- and C-terminal sequencing by MALDI-TOF
  • N-terminal sequencing by Edman (partner laboratory)
  • Lysine clipping analysis of mAB by CEX-UV
  • Molecular weight determination by LC-ESI-TOF
  • Molecular weight determination by MALDI-TOF
  • Targeted modifications: Deamidation, Glycation, PEGylation, Phosphorylation, Oxidation, etc.
  • Drug-to-antibody ratio (DAR) for ADCs
  • Accessible by peptide mapping by (LC-)MALDI-TOF and Molecular weight determination by LC-ESI-TOF and MALDI-TOF
  • Access to galactosylation, sialylation, mannosylation, afucosylation, bisecting GlcNAc, alpha-galactose, presence of O-glycosylation, etc. by the following methods
  • Molecular weight determination by LC-ESI-TOF
  • N- and O-glycan analysis by HILIC-FLD, MALDI-TOF, and HPAEC-PAD
  • Monosaccharide analysis by HPAEC-PAD
  • Sialic acid analysis by HPAEC-PAD and CE-UV
  • Glycan linkage analysis by GC-MS
  • Access to deamidation, amidation, pyroglutamate, lysine clipping at C-terminus, or protein fragments
  • Ion exchange chromatography (IEX-UV)
  • Isoelectric focusing (IEF)
  • Capillary isoelectric focusing (cIEF)
  • Capillary zone electrophoresis (CZE)
  • Access to protein fragments, level of impurities, and aggregation
  • Size exclusion chromatography (SEC-UV)
  • Capillary gel electrophoresis (CGE) / capillary electrophoresis sodium dodecyl sulfate (CE-SDS)
  • (2D-)SDS-PAGE