AAV vectors

The development of cell and gene based therapeutics like AAV (adeno-associated virus) vectors has gained significant importance in recent years. We provide a comprehensive list of analytical methods for characterization of different product characteristics.

  • Genomic particle titer by (Droplet) Digital PCR
  • Total particle titer by AAV capsid ELISA
  • Total protein by UV280nm
  • Total protein by Bradford

  • Host cell protein (HCP) ELISA
  • Quantification of specific HCPs by mass spectrometry (AQUA)
  • Host cell DNA
  • Next generation sequencing (NGS) services
  • Visible particles
  • Foreign subvisible particles

  • Aggregates by size exclusion chromatography (SEC)
  • Capsid load by transmission electron microscopy (TEM) or HPLC
  • SNPs, duplications, E.coli DNA and other DNA contaminations

  • Analytical ultracentrifugation (AUC)
  • Amino acid sequence confirmation by LC-MS peptide mapping
  • Quantitative analysis of posttranslational modifications by LC-MS peptide mapping (e.g. N-glycosylation, deamidation or oxidation)
  • Ratio of capsid proteins by LC-MS peptide mapping

Get in contact and learn more about our analytical capabilities.